Cytochrome c oxidase copper chaperone, COX17 

Description:   Cox17 is the candidate copper metallochaperone for delivery of Cu(I) ions to the mitochondrion for assembly of cytochrome c oxidase [1, 2]. Binds copper ions and delivers them to the Cu(A) site of COX [2]. Both the reduced and oxidized forms of Cox17 were found to promote the survival of NIH3T3 cells in serum-free medium [3]. May have influence on insulin secretion [4].

Species:    Porcine

Amino acid sequence:    PGLAAAIPAP PESQEKKPLK PCCACPETKK ARDACIIEKG EEHCGHLIEA HKECMRALGF KI

Modified amino acids:    Disulfide bridges between Cys(22)-Cys(25), Cys(23)-Cys(54), and Cys(35)-Cys(44). 

Molecular mass:    6688.9

Purity:    >98% by HPLC

Solubility:   Buffer

Storage:   Freezer (-20°C). Protect from moisture. Following reconstitution, aliquot and freeze (-20°C).

References:   [1]  Glerum, D.M., Shtanko, A., Tzagoloff, A.Characterization of COX17, a yeast gene involved in copper metabolism and assembly of cytochrome oxidase. Journal of Biological Chemistry. 271(24):14504-9, 1996 Jun 14. [2] Heaton, D.N., George, G.N., Winge, D.R. The mitochondrial copper metallochaperone Cox17 exists as an oligomeric, polycopper complex. Biochemistry. 40(3):743-51, 2001 Jan 23. [3]   Takenouchi T. Fujimoto M. Shimamoto A. Munekata E. Isolation and characterization of Cox17p from porcine heart by determining its survival-promoting activity in NIH3T3 cells. Biochimica et Biophysica Acta. 1472(3):498-508, 1999. [4] Chen ZW. Bergman T. Ostenson CG. Efendic S. Mutt V. Jornvall H. Characterization of dopuin, a polypeptide with special residue distributions. European Journal of Biochemistry. 249(2):518-22, 1997.