Reference [2]
[2] Andon B, Barbosa J, Sanz-Nebot V. (2006) Electrophoresis 27, 3661-3670.
Separation and characterization of rabbit liver apothioneins by capillary electrophoresis coupled to electrospray ionization time-of-flight mass spectrometry.
The present study establishes a method for the
separation and characterization of rabbit liver metallothionein (MT) subisoforms
by capillary electrophoresis coupled to electrospray ionization time-of-flight
mass spectrometry (CE-ESI-TOF-MS) via a sheath-flow interface. Directly
coupled-CE-MS enables the extraction of specific molecular weight information
and thereby facilitates the identification of peaks when no reference materials
are available, as in the case of MT subisoforms. The analysis described here
revealed the presence of the apothioneins MT-1a, MT-2d, and MT-2e, belonging to
MT-I sample, and MT-2a, MT-2b, and MT-2c, belonging to MT-II. Several
non-N-acetylated forms were also detected as traces appearing with their
respective acetylated forms in both samples. Similar results were found when
MALDI-TOF experiments were performed, identifying all the sequenced rabbit liver
MTs as apo-MT-forms, as in the CE-ESI-MS coupling.
PubMed: PMID: 16927421