Zn₇-Metallothionein-1A/2B mixture

Synonyms:    Zn₇-MT-1A/2B mixture.

Description:    Mixture of two metallothionein zinc-complexes from rabbit liver [1]. The thiolates of the Cys residues are chelated with seven  zinc ions. (One of the apoproteins is identical to MT-I, and the other to MT-II of those in the preparation from Sigma [2]).

Species:    Rabbit

Amino acid sequences:    

MT-1A:

*MDPNCSCATG NSCTCASSCK CKECKCTSCK KSCCSCCPAG CTKCAQGCIC KGASDKCSCC A

MT-2B:

*MDPNCSCATG DSCTCASSCK CKECKCTSCK KSCCSCCPAG CTKCAQGCIC KGASDKCSCC A

Differences in the sequences are indicated in purple

Modified amino acids:    *) Met-1 is acetylated, and the thiolates of the Cys residues are chelated with zinc.

Purity: About 60% of Zn₇-MT1A and 40% of Zn₇-MT2B.

Molecular masses:

Zn₇MT1A: 6583.07

Zn₇MT2B: 6584.05

Form:    Lyophilized.

Solubility:    A suitable buffer that does not disrupt the structure of the proteins.

Storage:     Freezer (-20° C). Protect from moisture. In solution at neutral pH and in the presence of oxygen metallothionein is sensitive to oxidation and solutions should not be stored. 

References:    [1] Hunziker PE, Kaur P, Wan M, Kanzig A. (1995) Primary structures of 7 metallothioneins from rabbit tissue, Biochem. J. 306, 265-270. [2] Andon B, Barbosa J, Sanz-Nebot V. (2006) Electrophoresis 27, 3661-3670.