Metallothionein-1A/2B mixture

Synonyms:    apo-MT-1A/2B mixture

Description:    Mixture of two metal-free apoproteins from rabbit liver [1]. One of them is identical to one of the apoproteins in MT-I preparation, and the other to one of the apoproteins in MT-II preparation from Sigma [2].

Species:    Rabbit

Amino acid sequences:    

MT-1A:

*MDPNCSCATG NSCTCASSCK CKECKCTSCK KSCCSCCPAG CTKCAQGCIC KGASDKCSCC A

MT-2B:

*MDPNCSCATG DSCTCASSCK CKECKCTSCK KSCCSCCPAG CTKCAQGCIC KGASDKCSCC A

Differences in the sequences are indicated in purple

Modified amino acids:    *) Met-1 acetylated

Purity: About 50:50 of each form

Molecular masses:

MT-1A: 6145.37

MT-2B: 6146.35

Form:    Lyophilized.

Solubility:    Buffer.

Storage:     Freezer (-20° C). Protect from moisture. In solution at neutral pH and in the presence of oxygen metallothionein is sensitive to oxidation and solutions should not be stored. 

References:    [1] Hunziker PE, Kaur P, Wan M, Kanzig A. (1995) Primary structures of 7 metallothioneins from rabbit tissue, Biochem. J. 306, 265-270. [2] Andon B, Barbosa J, Sanz-Nebot V. (2006) Electrophoresis 27, 3661-3670.