Metallothionein-2A, carboxyamidomethylated

Synonyms:    CAM-MT-2A; CAM-MT-II

Description:    Highly pure metal-free protein from rabbit liver [1] with carboxyamidomethylated cysteines. Coupling of an acetamide group to Cys residues makes them stable to oxidation, but also eliminates the metal-binding properties of the protein making it a suitable reference protein for control experiments.

Species:    Rabbit

Amino acid sequence:    MDPNCSCAAA GDSCTCANSC TCKACKCTSC KKSCCSCCPP GCAKCAQGCI CKGASDKCSC CA

Modified amino acids:    All 20 Cys residues are carboxyamidomethylated; Met-1 acetylated.

Purity:    >98% by HPLC and mass spectrometry.

Molecular mass: 7266.38

Form:    Lyophilized.

Solubility:    Buffer

Storage:     Freezer (-20° C). Protect from moisture. In solution at neutral pH and in the presence of oxygen metallothionein is sensitive to oxidation and solutions should not be stored. Carboxyamidomethylated metallothionein, however, is relatively stable.

References:    [1] Hunziker PE, Kaur P, Wan M, Kanzig A. (1995) Primary structures of 7 metallothioneins from rabbit tissue, Biochem. J. 306, 265-270.