Metallothionein-4

Synonyms:    apo-MT-4

Description:    Metal-free apoprotein, binds zinc and copper. Could play a special role in regulating zinc metabolism during the differentiation of stratified epithelia. Expressed exclusively in stratified squamous epithelia associated with oral epithelia, esophagus, upper stomach, tail, footpads and neonatal skin.

Species:    Mouse

Amino acid sequence:    MDPGECTCMS GGICICGDNC KCTTCSCKTC RKSCCPCCPP GCAKCARGCI CKGGSDKCSC CP

Modified amino acids:    None

Molecular mass:    6276.59

Purity:    >98% by HPLC (see HPLC analysis)

Form:    Lyophilized.

Solubility:    Suitable buffer that protects the protein from oxidation.

Storage:     Freezer (-20° C). Protect from moisture. In solution at neutral pH and in the presence of oxygen metallothionein is sensitive to oxidation and solutions should not be stored.

References:   Quaife, C. J., Findley, S. D., Erickson, J. C., Froelick, G. J., Kelly, E. J., Zambrowicz, B. P. & Palmiter, R. D. (1994) Induction of a new metallothionein isoform (MT-IV) occurs during differentiation of stratified squamous epithelia, Biochemistry. 33, 7250-7259.