PEC-60

Synonyms:    PEC-60; 60-residue polypeptide with N-terminal Glu and C-terminal Cys

Description: A 60-residue intestinal hormone-like peptide. Inhibits the glucoseinduced insulin secretion from perfused pancreas [1] and plays a role in the immune system. Synthesized in duodenal goblet cells and in monocytes in bone marrow and blood [2]. Increases dopamine release and activates Na+, K+-ATPase in brain [3, 4]. Contains a Kazal-like domain, but does not inhibit trypsin.

Biological function: Largely unknown.

Uses: Functional, biochemical and physico-chemical studies.

Species:   Porcine

Amino acid sequence:  EKQVFSRMPICEHMTE SPDCSRIYDPVCGTDGVTYESE CKLCLARIENKQDIQIVKDGEC

Modified amino acids:    Disulfide bridges between Cys(11)-Cys(42), Cys(20)-Cys(39), and Cys(28)-Cys(60).

Purity:    >98% by HPLC and MS.

Form:    Lyophilized solid.

Solubility:    Buffer.

Storage:    Freezer (-20°C). Protect from moisture. Following reconstitution, aliquot and freeze (-20°C).

References:   [1] Agerberth, B., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8590.  [2] Rimodini, R., et al. 1994. Neurosci. Lett. 177, 53. [3] Metsis, M., et al. 1992. J. Biol. Chem. 267, 19829. [4] Kairane, C., et al. 1994. FEBS Lett. 345, 1.