Zn₇-Metallothionein-3

Synonyms:    Zn₇MT-3; MT-3; GIF - Growth Inhibitory Factor;  

Description:    Zinc binding protein expressed in the mammalian brain, inhibits growth of cultured neurons and is specifically down-regulated in Alzheimer's disease. Noncovalent complex with 7 zinc. Recombinant protein.

Species:    Human.

Amino acid sequence:    MDPETCPCPS GGSCTCADSC KCEGCKCTSC KKSCCSCCPA ECEKCAKDCV CKGGEAAEAEAEKCSCCQ

Modified amino acids:    All Cys-residues are in the form of  thiolates, which participate in the binding of seven Zn ²⁺ ions.

Molecular mass:    7364.9

Form:    Lyophilized. 

Purity:    Peptide purity >98% by HPLC. Contains approx. 80 % of Zn₇MT-3 as the major metalloform and approx. 10 % of Zn₆MT-3, and 10 % of Zn₈MT-3 as minor metalloforms  by electrospray mass spectrometry.

Solubility:    Buffer.

Storage:    Freezer (-20° C). Protect from moisture. In solution at neutral pH and in the presence of oxygen metallothionein is sensitive to oxidation and solutions should not be stored.

References:   [1] Uchida, Y., et al. 1991. Neuron 7, 337; [2] Palmiter, R. et al. 1992. PNAS 89 , 6333; [3]  Yu, W.H., et al. 2002. Brain Res. 894, 37.